Table 1 Subthreshold and chronic unpredictable stress procedures.
Stressors | ||||
|---|---|---|---|---|
AM | PM | |||
Day 1 | 2-h restraint | 15-min tail pinch | SUSa (3 days) | CUSa (10 days) |
Day 2 | 24-h constant light | 2-h restraint | ||
Day 3 | 2-h restraint | 24-h 45° cage tilt and wet bedding | ||
Day 4 | 10-min inescapable shock (0.3 mA, 2-s duration, at random intervals with an average of 16 s) | 2-h restraint | ||
Day 5 | 2-h restraint | 30-min elevated platform | ||
Day 6 | 15-min tail pinch | 2-h restraint | ||
Day 7 | 2-h restraint | 24-h constant light | ||
Day 8 | 24-h 45° cage tilt and wet bedding | 2-h restraint | ||
Day 9 | 2-h restraint | 10-min inescapable shock (0.3 mA, 2-s duration, at random intervals with an average of 16 s) | ||
Day 10 | 30-min elevated platform | 2-h restraint | ||