Table 1 Subthreshold and chronic unpredictable stress procedures.

From: Increased intrinsic and synaptic excitability of hypothalamic POMC neurons underlies chronic stress-induced behavioral deficits

 

Stressors

 

AM

PM

Day 1

2-h restraint

15-min tail pinch

SUSa (3 days)

CUSa (10 days)

Day 2

24-h constant light

2-h restraint

Day 3

2-h restraint

24-h 45° cage tilt and wet bedding

Day 4

10-min inescapable shock (0.3 mA, 2-s duration, at random intervals with an average of 16 s)

2-h restraint

 

Day 5

2-h restraint

30-min elevated platform

Day 6

15-min tail pinch

2-h restraint

Day 7

2-h restraint

24-h constant light

Day 8

24-h 45° cage tilt and wet bedding

2-h restraint

Day 9

2-h restraint

10-min inescapable shock (0.3 mA, 2-s duration, at random intervals with an average of 16 s)

Day 10

30-min elevated platform

2-h restraint

  1. aSUS subthreshold unpredictable stress, CUS chronic unpredictable stress.