Fig. 2: Chemogenetic inhibition of PL pyramidal neurons ameliorates repetitive behavior and social novelty impairment.
From: Divergent projections of the prelimbic cortex mediate autism- and anxiety-like behaviors
a Diagram of virus injection and experimental procedure in WT and Tmem74−/− mice. b The injecting site of pAAV2/9-CaMKIIα-hM4D(Gi)-mCherry-3FLAG (AAV-CaMKIIα-hM4D(Gi)-mCherry) and validation of hM4D(Gi)-mCherry in PL pyramidal neurons in Tmem74−/− mice. c Representative spike firing of PL pyramidal neurons in hM4D(Gi) expression brain slices incubated with CNO (10 μmol/L). d Quantification of the AP frequency by current injections from 0 to 300 pA (stepped by 20 pA) (n = 9 cells from 3 WT- hM4Di+Veh mice, n = 11 cells from 3 Tmem74−/−-hM4Di+Veh mice, n = 7 cells from 3 Tmem74−/−-hM4Di+CNO mice). e, f The time spent on grooming (e) and the percentage of buried marbles (f) in mice (e, n = 10 WT-hM4Di+Veh mice, n = 12 Tmem74−/−-hM4Di+Veh mice, n = 11 Tmem74−/−-hM4Di+CNO mice; f, n = 10 WT-hM4Di+Veh mice, n = 12 Tmem74−/−-hM4Di+Veh mice, n = 12 Tmem74−/−-hM4Di+CNO mice). g–j The interaction time (g) and social preference index (h) in all groups during phase 2, and the interaction time (i) and social novelty index (j) during phase 3 from three-chamber test (n = 13 WT-hM4Di+Veh mice, n = 14 Tmem74−/−-hM4Di+Veh mice, n = 16 Tmem74−/−-hM4Di+CNO mice). Data were presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001; ns not significant. Two-way ANOVA followed by Bonferroni’s post hoc test for d; One-way ANOVA followed by Tukey’s post hoc test for e, f; Two-way ANOVA followed by Bonferroni’s post hoc test for g; One-way ANOVA followed by Bonferroni’s post hoc test for h; Two-way ANOVA followed by Sidak’s post hoc test for i; one-way ANOVA followed by Sidak’s post hoc test for j.
