Fig. 5: Anatomically distinct subpopulations of PL pyramidal neurons project to the STR and BLA. | Molecular Psychiatry

Fig. 5: Anatomically distinct subpopulations of PL pyramidal neurons project to the STR and BLA.

From: Divergent projections of the prelimbic cortex mediate autism- and anxiety-like behaviors

Fig. 5: Anatomically distinct subpopulations of PL pyramidal neurons project to the STR and BLA.The alternative text for this image may have been generated using AI.

a, b Schematic and histology of AAV2-Retro-EF1α-DIO-EGFP-WPRE-hGHpA (rAAV-DIO-EGFP, green) injection into dSTR and AAV2-Retro-EF1a-DIO-mCherry-WPRE-hGHpA (rAAV-DIO-mCherry, red) injection into BLA in CaMKIIα-Cre mice. c A coronal brain slice (2.2 mm anterior bregma) of PL neurons in CaMKIIα-Cre mice after rAAV-DIO-EGFP injection into dSTR and rAAV-DIO-mCherry injection into BLA. d Quantification of number of PL pyramidal neurons labeled with green from dSTR injections and red from BLA injections (n = 5 in each group, and two views of slices were provided for each mouse). e Quantification of the medial/lateral pyramidal neurons distribution of PL–STR (green) and PL–BLA (Red). Shading denotes SEM (The two groups are distributed differently, p = 0.0118). f An overlapping Venn diagram of the PL–dSTR neurons and PL–BLA neurons (neuron overlap:3.56%). Data were presented as means ± SEM. *P < 0.05; ns not significant. Unpaired two-tailed Student’s t-test for d; Kolmogorov-Smirnov test for e.

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