Fig. 6: Both flTCF7L2 and dnTCF7L2 are required for mouse USVs.
a USV measurement in exon1 fl/+;Vglut2-Cre/+ (n = 15) at P7. The exon1 fl/+ mice (n = 19) served as controls. b The expression level of TCF7L2 in the midbrain of exon1 fl/+;Vglut2-Cre/+ (n = 4) and exon1 fl/+ (n = 4) mice at P7. GAPDH, loading control. c Different isoforms of Tcf7l2 in mouse brain was identified by nanopore sequencing and the isoform percentages were illustrated. d IGV visualization of 10 alternative exon 5’. Red triangles illustrate the 5’ splicing sites of these alternative exon 5’. e Transcripts containing exon 5’ were detected in neuronal but not non-neuronal tissues by RT-PCR at P7, 1.5-month, and 3-month-old mice. f DnTCF7L2 are expressed in neuronal but not non-neuronal tissues at P7. Expression of dnTCF7L2 in the midbrains in the indicated genotypes at P7 (g). The data summary was shown in (h) (n = 3). i USV measurement in the indicated genotypes at P7. In a, b, h, and i, the value are presented as mean ± SD. N.S., no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001, t-test or ANOVA, SPSS. In i, +/+ (n = 10), Tcf7l2ΔEx5’/+ (n = 19), Tcf7l2ΔEx5’/ΔEx5’ (n = 9).
