Fig. 3: The single-cell RNA-seq of E11.5 AGM and E10.5 YS shows that the global transcriptomic changes encoding reduced ISR activation, IRES-mediated translation, and increased energy production in BTBR/R mice. | Molecular Psychiatry

Fig. 3: The single-cell RNA-seq of E11.5 AGM and E10.5 YS shows that the global transcriptomic changes encoding reduced ISR activation, IRES-mediated translation, and increased energy production in BTBR/R mice.

From: An old model with new insights: endogenous retroviruses drive the evolvement toward ASD susceptibility and hijack transcription machinery during development

Fig. 3

a Bubble plot of DEG in AGM cell clusters. Progenitor cells across definitive hematopoiesis include Adgrg6+ hemogenic endothelium (HE) (cluster 6, C6), Pecam1+ pro-hematopoietic stem cells (ProHSC) (cluster 12, C12), Pecam1- pre-hematopoietic stem cells type I (PreHSC I) (C12), pre-hematopoietic stem cells type II (PreHSC II) (cluster 17, C17); differentiated cell type includes Adgrg6- HE (C6), cluster 7 and cluster 9 (See Supplementary Fig. 5 for cell clustering by UMAP). b Bubble plot of DEG in YS cell clusters indicated widespread changes centering on the viral infection process. Progenitor cells across definitive hematopoiesis include Cdh5+ erythro-myeloid progenitor (EMP) (cluster 0, C0), Cdh5- EMP (C0), erythroid progenitors (EP) (cluster 3, C3), myeloid progenitors (MP) (cluster 5, C5); differentiated cell type includes hematopoietic cell (C3), microglia (cluster 13, C13), cluster 1, cluster 2, cluster 4 and cluster 9. All the DEGs had an FDR value of <0.05 with an average log fold-change (avg_ln FC) ≥ 0.25. Genes increased or decreased in BTBR/R compared to B6 were shown in pink or light blue, respectively. c The expression of key ISR mediator, Atf4 and its downstream effectors, Atf3 and Ppp1r15a in AGM Adgrg6+ HE. Atf4, p < 0.0001; Atf3, p < 0.0001; Ppp1r15a, p < 0.0001). d The expression of key ISR mediator, Atf4 and its downstream effectors, Atf3 and PPP1r15a in YS Cdh5+ EMP. Atf4, non-significant; Atf3, p < 0.0001; Ppp1r15a, p < 0.0001). e The expression of Ribosomal protein facilitating virus infection in AGM Adgrg6+ HE. Rpl29, p < 0.0001; Rps3a1, p < 0.0001; Rps2, p < 0.0001). f The expression of Ribosomal protein facilitating virus infection in YS Cdh5+ EMP. Rpl29, p < 0.0001; Rps3a1, p < 0.0001; Rps2, p < 0.0001).

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