Fig. 5: Network activity and E/I ratio are reduced in the limbic system of Pcdh19 mosaic mice.
a Top left, in vivo recordings experimental planning. Top right, electrodes position in limbic system regions and representative extracellular recordings from which single spike units and LFP signals were extrapolated. Bottom, spike sorting process used to extrapolate single spike unit activity (ISI = interspike interval). b Representative traces of neuronal extracellular activity (left panel) and MFR (right panels) in different limbic system regions of mosaic mice (Pcdh19fl/y Syn1-Cre, Mosaic) and controls (Pcdh19fl/y, Ctrl) (N, 5-7 recording sessions/brain region/mouse, 2 mice/genotype; Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001). c Classification of neurons into excitatory and inhibitory according to the duration of AP depolarization and hyperpolarization phases. Hierarchical clustering allowed sorting of neurons from Pcdh19 mosaic and control mice as in b (red, excitatory neurons; blue, inhibitory neurons; grey, unclassified neurons). d Number of excitatory and inhibitory neurons and ratio in limbic system regions of Mosaic and Ctrl mice as in b (N, 5-7 recording sessions/brain region/mouse, 2 mice/genotype). e MFR of excitatory and inhibitory neurons and MFR ratio in limbic system regions of Mosaic and Ctrl mice as in b (N, 5-7 recording sessions/brain region/mouse, 2 mice/genotype; Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001). All data (b, d, e) are shown as means ± SEM. See also Supplementary Table 5.
