Fig. 5: rNLS8 mice develop reactive astrogliosis and altered astrocytic gene expression, which are not ameliorated by knockdown of Chop. | Molecular Psychiatry

Fig. 5: rNLS8 mice develop reactive astrogliosis and altered astrocytic gene expression, which are not ameliorated by knockdown of Chop.

From: Early activation of cellular stress and death pathways caused by cytoplasmic TDP-43 in the rNLS8 mouse model of ALS and FTD

Fig. 5

Representative IF images for pan-TDP-43 (human+mouse) (green), reactive-astrocytic marker GFAP (red), and the nuclear marker DAPI (blue) in the layer V of cortex (A) or the ventral horn of spinal cord (D) of mice at 6 weeks off Dox (WOD). Scale bars represent 50 µm. Cell counting for GFAP positively labeled astrocytes in the cortex (B) and spinal cord (E) of treated mice (n = 3). Quantification of IF for GFAP intensity in the cortex (C) and spinal cord (F) of treated mice at 6 WOD (n = 3). qPCR analysis of astrocytic gene expression in the cortex (G) and spinal cord (H) of Chop ASO-treated mice at 6 WOD (n = 4). Data are normalised to Actb as the housekeeping gene. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.

Back to article page