Fig. 5: Elevated levels of mitochondrial superoxide in AS NPCs.

A A Representative FACS-derived histogram of the MitoSOX fluorescence intensity, measuring the mitochondrial superoxide levels of WT and AS cultured NPCs. The dashed vertical line depicts the threshold for “high” MitoSOX fluorescence (see C). B AS NPCs show a higher mean fluorescence intensity (MFI) of MitoSOX than WT NPCs. C AS NPCs consist a higher percentage of cells with high fluorescence intensity of MitoSOX. N = 4 for each group. For all panels, data are presented in means ± SEM (*p < 0.05 in t-test).