Fig. 8: GSH replenishment by GSH-EE application does not restore the enhanced mitochondrial membrane potential (ΔΨm) in AS NPCs. | Molecular Psychiatry

Fig. 8: GSH replenishment by GSH-EE application does not restore the enhanced mitochondrial membrane potential (ΔΨm) in AS NPCs.

From: Elevated ROS levels during the early development of Angelman syndrome alter the apoptotic capacity of the developing neural precursor cells

Fig. 8

A AS-vehicle NPCs show enhanced ΔΨm compared to WT-vehicle NPCs, as defined by the TMRE- mean fluorescence intensity (MFI). GSH-EE-treated AS and WT NPCs do not significantly differ in TMRE MFI, yet, the treatment effect does not reach significance. B Representative dual-parameter FACS plots of Mito Tracker Green and TMRE. The rectangular boxes represent the two populations which were arbitrarily defined as NPCs with high (↑ΔΨm) and low ΔΨm (↓ΔΨm). C Unlike AS-vehicle, AS-treated NPCs do not display a higher ratio of cells with high ΔΨm than WT-treated NPCs, yet, the treatment effect is insignificant. N  =  4 for each group. For all panels, data are presented in means ± SEM (ns= non-significant, *p < 0.05 in two-way ANOVA).

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