Fig. 2: Fetal CBD exposure increases thermal sensitivity in male mice, but not female mice.

Fetal CBD exposure does not affect latency to response to thermal stimulus in the Hargreaves test in wildtype or TRPV1^KO/KO female mice (A), and over the estrus cycle (B). Fetal CBD exposure decreases latency to response in wild-type CBD-exposed male mice (11.58 ± 0.64 s for vehicle-exposed vs 6.87 ± 3.27 s, P = 4.993E−8, t-test), but does not affect latency response in TRPV1^KO/KO mice (11.089 ± 0.649 s vehicle-exposed, vs. 12.429 ± 1.610 s CBD-exposed P = 0.453, t-test) (C). Graphs show fetal CBD exposure does not affect time in the center zone, time moving, or time still in the Open field test in 6-week-old female (D), or male offspring (E). Graphs show fetal CBD exposure does not affect time in open area, near zone or far zone in the Light Dark Box in 8-week-old female (F) or male (G) offspring. The elevated zero maze shows that fetal CBD exposure does not affect time in closed or open areas in 10-week-old female (H), and male (I) offspring. Fetal CBD exposure did not affect zone crossings in female (J), nor male offspring (K) in the elevated zero maze. Error bars represent the S.E.M. All mean values of measurements, S.E.M., and p values from t-tests are reported in Table 2.