Fig. 2: LHb integrity is necessary for cue discrimination during Pavlovian conditioning.
From: Plasticity of neuronal dynamics in the lateral habenula for cue-punishment associative learning
a Experimental protocol of the behavioral setup for the Pavlovian conditioning task in awake head-restrained mice. b Left, schematic representing the structure of conditioning; right, example pictures of the behavioral responses during the task c Time course of the average eye area changes across CS+ trials during habituation (H) and conditioning (C) (nmice=10). d Box-plot of the discrimination score during habituation and conditioning (nmice=10; paired t test, t9 = 4.92, ***p < 0.001). e Top, schematic of optogenetic inhibition and representative coronal section of Jaws expression in the LHb and fiber track (scale bar: 200 µm); bottom, schematic of the experimental protocol for optogenetic LHb inhibition. f Box-plots of the discrimination score for GFP mice (nmice=7; paired t test, t6 = 3.20, *p = 0.019) and Jaws mice (nmice=10; paired t test, t9 = 0.059, p = 0.954).
