Fig. 3: Single neuron inhibitory and excitatory responses adapt during associative learning.
From: Plasticity of neuronal dynamics in the lateral habenula for cue-punishment associative learning
a Schematic of the experimental preparation for the LHb two-photon calcium imaging, example coronal histological section of GRIN lens positioning above LHb (scale bar: 200 µm), GCaMP6f expression on LHb neurons (scale bar: 100 µm) and staining of the EAAC1 glutamatergic marker (scale bar: 30 µm). b Schematic representation of the experimental setting for two-photon calcium imaging recordings in head-restrained awake mice (left) and standard deviation projection of an example field of view (FOV) (right). c Example of longitudinal tracking of the same FOV across habituation and conditioning sessions (top) and average response of the indicated neuron (arrowhead; bottom; scale bar: 10 ΔF/F0 %, 3 s). d Heatmaps of the average ΔF/F0 response of single neurons during habituation (left) and conditioning (right) (scale bar: 1 s). e Scatter plot of the area under the curve (AUC) during CS+ and CS- for habituation (white dots) and conditioning (red dots) (F(1,674) = 86.76, *p < 0.0001, nmice = 12, nneurons = 339). f Left, contingency table of the proportion of neurons statistically inhibited, not responding or excited during CS+ presentation (Χ2 = 8.10, *p = 0.018); right, time-course of the grand-average responses to CS+ trials during habituation and conditioning trials for the excited (top, nneurons = 118) and inhibited neurons (bottom, nneurons = 45). g Box-plot of the population average AUC response during CS+ presentation for the excited (left; paired t-test, t117 = 9.08, ***p < 0.0001) and inhibited neurons (right; paired t-test, t44 = 6.70, ** *p < 0.0001). h Box-plot of the accuracy score for the excited and inhibited neurons (ncells: excited=47; inhibited=10; Two Way ANOVA RM and Sidak’s multiple comparison test, shuffle vs score F(1,55) = 93.84, *p < 0.0001).
