Fig. 2: Rapamycin treatment attenuates chronic stress-induced increase in serum cf-mtDNA and behavior deficits. | Molecular Psychiatry

Fig. 2: Rapamycin treatment attenuates chronic stress-induced increase in serum cf-mtDNA and behavior deficits.

From: Activation of cell-free mtDNA-TLR9 signaling mediates chronic stress-induced social behavior deficits

Fig. 2

A, B Expression of serum mtDNA genes (Cox1 and 12 S) in no stress (NS) or restraint stress (RS) exposed mice treated with PBS or Rapamycin (RAPA). Cox1; Two-way ANOVA, stress (F (1, 12) = 6.493, p = 0.0256); treatment (F (1, 12) = 35.32, p < 0.0001) and interaction (stress x treatment) (F (1, 12) = 15.55, P = 0.0019); **p < 0.01 (vs PBS + NS) and ####p < 0.0001 (vs PBS + RS), n = 4 per group. 12 S; Two-way ANOVA, stress (F (1, 12) = 5.953, p = 0.0312); treatment (F (1, 12) = 8.141, p = 0.0145) and interaction (stress x treatment) (F (1, 12) = 6.258, p = 0.0278); *p < 0.05 (vs PBS + NS) and #p < 0.05 (vs PBS + RS), n = 4 per group. C, D RAPA treatment attenuated RS-induced deficits in social behavior. C Time in chamber in the three-chamber social interaction test. Two-way ANOVA, chamber (F (2, 93) = 172.3, p < 0.0001); interaction (chamber X treatment) (F (6, 93) = 6.635, p < 0.0001). ****p < 0.0001 (mouse chamber vs empty chamber); n = 8–9 per group. D Reciprocal social interaction test; Two-way ANOVA, treatment (F (1, 31) = 8.976, p = 0.0053); *p < 0.05 vs PBS + NS; n = 8–9 per group.

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