Fig. 1: Experimental background and overview.
From: Molecular neurobiology of loss: a role for basolateral amygdala extracellular matrix
A Example of environmental enrichment (EE), and timeline for standard enrichment removal (ER) protocol. B Representative images of the BLA from initial Fos IHC screen and quantification (SH n = 9, EE n = 9, ER n = 8; scale bar = 100 µm). C Outline for omics and follow-up studies. Experiments 2 and 3 utilized multi-omics in tandem with a number of complementary bioinformatics analyses to explore the molecular landscape of the BLA following ER. Experiments 4 and 5 further explored the candidate mechanisms that emerged from these analyses, as well as their role in ER behavioral phenotypes, to expand our understanding of novel mechanisms that underlie loss. Experiment 6 explores manipulating the molecular mechanisms identified here in an attempt to rescue the loss-like behavioral phenotypes generated by ER. * = p < 0.05.
