Fig. 6: Spontaneous anxiety-like behaviors were increased in GABAergic neuron-specific AMPK knockout mice.

a AMPK knockout in GABAergic neurons (left, AMPK KOGABA) and the representative result of gene validation (right). In the OFT, (b) representative traces and (c) the time (left) and distance (right) mice spent in the center area. In the EPM, (d) representative traces and (e) the duration (left) and entries (right) in the open arms. (f) In the LDT, time (left) spent in the light box and the latency (right) to enter the light box. g Schematic illustration of the AMPK-dependent anxiolytic effect of metformin by mPFC GABAergic microcircuit action. Ns, *P ˂ 0.05 by unpaired Student’s t test.