Fig. 2: Brain components generated by group independent component analysis (ICA) applied to cohort 2: NR1 offspring at the age of 8 weeks together with 8w-controls.

Group independent component analysis (ICA) was applied to all mice from cohort 2, consisting of 15 NR1 offspring at the age of 8 weeks and and 15 controls at the age of 8 weeks. Resting state networks common to all subjects from cohort 2 (n = 30) were identified using temporal-concatenation ICA as implemented in Functional Magnetic Resonance Imaging of the Brain Software Library Multivariate Exploratory Linear Optimized Decomposition of Independent Components (FSL MELODIC) [31, 33]. Anatomically plausible components that were generated by group ICA are displayed as spatial color-coded z-maps (red-yellow heat maps) onto the Allen Mouse Brain Atlas (AMBA). Components are arranged according to anatomical mouse brain structures after visual correlation with AMBA brain regions modified after previously published component grouping [29, 71]. Components were found in all main anatomical regions and were subsequently used for group comparison of functional connectivity between NR1 offspring and controls (see Figs. 3–5). Anatomical labels: DG = dentate gyrus; Sub = subiculum; Amy = amygdala; aCing = anterior cingulate area; VMN = thalamic ventromedial nucleus; LGN = thalamic lateral geniculate nucleus; MDN = medial dorsal nucleus; LPN = lateral posterior nucleus; VPL = thalamic ventral posterolateral nucleus; VAN = ventral anterior nucleus; AN = anterior nuclear group of the thalamus; SS = primary somatosensory area; vAUD = ventral auditory area; MO = pimary motor area; VIS = primary and anterolateral visual area; RSP = retrosplenial cortex; Ent = entorhinal area; Ent = lateral part of the entorhinal area; OLF = main olfactory bulb; PIR = piriform area; CP = caudate putamen; GP = globus pallidus; MSN = medial septal nucleus area of pallidum; Acc = nucleus accumbens; HTh = Hypothalamus; PAG = periaqueductal gray; SC = superior colliculus; MRN = midbrain.