Fig. 3: SHLP2 is localized in mitochondrial complex 1 and co-expressed with mitochondrial genes.
From: A naturally occurring variant of SHLP2 is a protective factor in Parkinson’s disease
A Mitochondrial localization of SHLP2-APEX. HEK293 cells were transfected with SHLP2-APEX or APEX control, fixed, and stained with anti-myc and anti-mitofilin antibodies to visualize APEX and mitochondria. Nuclei were stained with Hoechst. Scale bars are 200μm. B Representative western blot image of IMM and OMM proteins following SHLP2-APEX pulldown (n = 3). C Analysis of the SHLP2-APEX proximity labeling to remove false positives and perform pathway enrichment. D SHLP2 interacting proteins in the mitochondrial complex 1 (blue) SHLP2-APEX pull down (Red) SHLP2 peptide column. SHLP2 co-expression genes’ (E) molecular function and (F) cellular location in iPSC-derived dopaminergic neurons (iPSCs-derived dopaminergic neurons generated from 18 individuals). The color code represents the adjusted p value scale. The count is the number of genes significantly correlated for each term. The x axis is gene ratio, which is the ratio of input genes that are annotated in a term.
