Fig. 3: In vivo alterations of ECM proteins in the hippocampi of Shank3∆11(−/−) mice. | Molecular Psychiatry

Fig. 3: In vivo alterations of ECM proteins in the hippocampi of Shank3∆11(−/−) mice.

From: Disrupted extracellular matrix and cell cycle genes in autism-associated Shank3 deficiency are targeted by lithium

Fig. 3: In vivo alterations of ECM proteins in the hippocampi of Shank3∆11(−/−) mice.The alternative text for this image may have been generated using AI.

a Collagens expressed in the cell cultures with p.adj < 0.1 and Map2. b Networks connecting collagens and other ECM related proteins, isolated from the STRING PPI network of all downregulated DEGs. c Pictogram of the tissue origin and genotype legend for d–g. Hippocampi of P35 Shank3∆11(−/−) and WT animals were used for protein isolation and subsequent WB analysis or coronary sectioned for IHC. d WB of COL1A1 and amounts relative (rel.) to actin (Z = 0, p** = 0.00781, n = 8 animals per genotype, paired wilcox rank test). e WB of COL2A1 and amounts rel. to actin (t = −1.015094369, df = 6, p = 0.349, n = 7 animals per genotype, paired two-sided t-test). f WB of COL4A1/2 and amounts rel. to actin (Z = 5, p = 0.0781, n = 8 animals per genotype, paired two-sided wilcox rank test). g Representative images from the CA1 hippocampal region stained for DAPI and COL4, and the analysis of the COL4 structures (number of structures: Z = 39, p = 0.427, n = 10 ROIs from 5 animals per genotype, paired two-sided wilcox rank test; area: Z = 294277, p = 0.131, n = 840(WT)/733(KO) structures from 5 animals per genotype, paired two-sided wilcox rank test; pixel intensity: Z = 209273, p**** < 0.0001, n = 840(WT)/733(KO) structures from 5 animals per genotype, paired two-sided wilcox rank test), scale bar = 100 µm, scale bar zoom = 25 µm.

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