Fig. 4: In vivo alterations of cell cycle proteins in the hippocampi of Shank3∆11(−/−) mice. | Molecular Psychiatry

Fig. 4: In vivo alterations of cell cycle proteins in the hippocampi of Shank3∆11(−/−) mice.

From: Disrupted extracellular matrix and cell cycle genes in autism-associated Shank3 deficiency are targeted by lithium

Fig. 4: In vivo alterations of cell cycle proteins in the hippocampi of Shank3∆11(−/−) mice.The alternative text for this image may have been generated using AI.

a Network connecting cell cycle related proteins, isolated from the STRING PPI network of all downregulated DEGs. PLK1 and KIF20A are labeled bold. b Ratios of marker positive cells to DAPI nuclei (GFAP: t = −0.743700079, df = 7.687872835, p = 0.479, n = 5 cell cultures per genotype, two-tailed t-test; IBA1: Z = 13, p = 1, n = 5 cell cultures per genotype, two-tailed wilcox rank test; MAP2: t = 0.672397714, df = 7.973620187, p = 0.52, n = 5 cell cultures per genotype, two-tailed t-test; OLIG2: t = 0.028751815, df = 4.643111315, p = 0.978, n = 5 cell cultures per genotype, two-tailed t-test). c DAPI nuclei per cell culture (Z = 14, p = 0.841, n = 5 cell cultures per genotype, two-tailed wilcox rank test). d Pictogram of the tissue origin and genotype legend for b, c, f–i. Hippocampi of P35 Shank3∆11(−/−) and WT animals were used for protein isolation and subsequent WB analysis or coronary sectioned for IHC. e Representative images from the CA1 hippocampal region stained for DAPI, NeuN and PLK1/KIF20A/pKIF20A, scale bar = 20 µm. f PLK1, KIF20A and pKIF20A log2 of signal intensity rel. to DAPI signal in single cells (PLK1: Z = 448875, p**** < 0.0001, n = 1092(WT)/1196(KO) from 5 animals per genotype, two-tailed wilcox rank test; KIF20A: Z = 464471, p**** < 0.0001, n = 1092 (KO)/1196(KO) from 5 animals per genotype, two-tailed wilcox rank test); pKIF20A: Z = 557197, p**** < 0.0001, n = 1308(KO)/1342(KO) from 5 animals per genotype, two-tailed wilcox rank test. g WB of TUBB and amounts rel. to actin (Z = 20, p = 0.844, n = 8 animals per genotype, paired two-sided wilcox rank test). h WB of GTP-TUB and amounts rel. to actin (t = 0.47115966, df = 7, p = 0.652, n = 8 animals per genotype, paired two-sided t-test). i Ratios between GTP-TUB to TUBB values of the same animals. (Z = 0, p = 0.00781, n = 8 animals per genotype, paired two-sided wilcox rank test).

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