Fig. 1: Isolation of activated neurons (AN) from Arc-TRAP/Arc-GFP mice following chronic social defeat (CSD) paradigm.

A Schematic representation of Arc-GFP mouse line. Transgenic animals contain two transgenes including a CreER^T2 under an activity-dependent Arc promoter (ArcCreER^T2) and Cre-dependent fusion reporter Sun1GFP. In the presence of tamoxifen (TAM), Cre-ER^T2 translocates to the nucleus allowing loxp site recombination and expression of the fusion nuclear membrane protein Sun1GFP. B Strategy for isolation of activated nuclei population. Nuclei were isolated from vHIP and PFC brain regions and sorted for GFP+ (AN) and GFP− populations using FANS. Nuclei isolation was performed from a pool of two mice per behavioral group. Sorted AN nuclei were processed for molecular assays such as nuclear RNA-seq. C Schematic diagram illustrating the experimental outline for CSDS (see more in Supplementary Fig. 1). Behavioral results prior to CSD experiments. Animals were segregated into control (black) and stressed (red) groups, based on their baseline behaviors of open field/eagle exploration (D) or social interactions with conspecifics (E). F Following CSD, the stressed group revealed a decrease in social interaction test compared to control (Kolmogorov–Smirnov test, p < 0.05). Animals with SI > 100 were considered as resilient and SI < 100 were considered as susceptible. G Stressed animals revealed significant weight gain compared to control animals (t-test, p < 0.05) on the 19th day. All error bars indicate ±SEM. Black circles represent individual non-stressed control mouse, while red circles represent individual stressed mouse.