Fig. 6: Characterization of Tau aggregates in 3xTg and Thy1-ApoE4/C/EBPβ Tg mice.
From: Thy1-ApoE4/C/EBPβ double transgenic mice act as a sporadic model with Alzheimer’s disease
A Diagram showing the isolation of soluble and insoluble Tau in the brain tissues of 3xTg and Thy1-ApoE4/C/EBPβ Tg mice. B Representative electron microscope images of Tau insoluble fraction extracted from the brains of 3xTg and Thy1-ApoE4/C/EBPβ Tg mice. Scale bar 100 nm. C ELISA assay of soluble Tau levels in the brains of 3xTg and Thy1-ApoE4/C/EBPβ Tg mice at different ages. (n = 3 mice, *p < 0.05, **p < 0.01). D ELISA assay of insoluble Tau levels in the brains of 3xTg and Thy1-ApoE4/C/EBPβ Tg mice at different ages. (n = 3 mice, *p < 0.05, **p < 0.01). Tau aggregates (2 μg) from the brains of 3xTg and Thy1-ApoE4/C/EBPβ Tg mice were transduced into HEK293-K18 cells stably expressing GFP-tagged Tau RD (repeat domain), After 24 h of transduction, the insoluble Tau inclusions in cells were imaged E and quantified F under microscopy. (***p < 0.01 as compared with vehicle). G Tau aggregates (5 μg) extracted from the brains of 3xTg and Thy1-ApoE4/C/EBPβ Tg mice were digested with Protease K (1 μg/ml) at different time points, and then immunoblotted with Tau antibody. Representative immunostaining images of AT8 H and TUNEL I, AEP and Tau N368 J in primary rat neurons treated with Tau aggregates (2 μg) extracted from the brains of 3xTg and Thy1-ApoE4/C/EBPβ Tg mice. Scale bar 20 μm.
