Fig. 1: Common ADs bidirectionally modulate fluid-phase endocytosis in the lower-therapeutic concentration range.
From: Antidepressant-induced membrane trafficking regulates blood-brain barrier permeability
A Representative images of 4 kDa FITC-dextran internalization in PC12 cells treated with fluvoxamine for 1 h. B Quantification of dextran internalization in fluvoxamine-treated cells, measured as total fluorescence signal per cell. Values are normalized to control. ****P < 0.0001, Kruskal–Wallis test. ****P < 0.0001, ns–not significant, Dunn’s multiple comparisons test. C Representative images of 4 kDa FITC-dextran internalization in PC12 cells treated with fluoxetine for 1 h. D Quantification of dextran internalization in fluoxetine-treated cells, measured as total fluorescence signal per cell. Values are normalized to control. ****P < 0.0001, Kruskal–Wallis test.****P < 0.0001, ***P < 0.001, **P < 0.01, Dunn’s multiple comparisons test. Quantification of 4 kDa FITC-dextran internalization in PC12 cells treated with vortioxetine (E), duloxetine (F), imipramine (G) mirtazapine (H), paroxetine (I), citalopram (J), escitalopram (K), and sertraline (L), measured as total fluorescence signal per cell. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns–not significant, Kruskal–Wallis test with Dunn’s multiple comparisons test. N ≥ 3 independent experiments, n = 30–45 cells.Scale bar, 5 μm.
