Fig. 2: Mean methylation rates of individual CG nucleotides within the promoter region of OPRK1 (subregion chr8:53250000-53253000) and localization of CG163 and DMR (CG34-CG38), which are more poorly methylated in BPD.

Both the new DMR localizing within the falling slope (5’) and CG163 within the rising slope (3’) of the methylation gap strengthen the effect of the promoter hypomethylation in BPD. Detail: enlarged view of the clustering CG34-38, each hypomethylated in BPD, building the DMR. Red dots: BPD, blue dots: HC.