Fig. 7: NitroSynapsin abrogates hypersynchronous burst activity in MHS cerebral organoids.

A Representative heat maps and single traces from Ctrl1 and MHS hiPSC-derived cerebral organoids in individual MEA wells at 3–4 months of age. B Representative raster plots of MEA recordings in Ctrl and MHS cerebral organoids. Boxes outline examples of network bursts. C Representative raster plots and heat maps of MEA recordings in Ctrl and MHS cerebral organoids after treatment with NitroSynapsin (NitroSyn). D–I Quantification of MEA mean firing rate, network burst frequency, and synchrony index. Each MHS patient’s cerebral organoids vs. each control shown in D, F and H; or grouped MHS patient cerebral organoids vs. controls shown in E, G and I. Data are mean ± SEM. Sample size is listed above bars from 6 to 7 separate cerebral organoids recorded for each genotype. *^,#,† p < 0.05, **^,##,††p < 0.01, ***^{,###, †††}p < 0.001, ****,^{####,††††}p < 0.0001 by ANOVA with Sidak’s post-hoc test for comparison to Ctrl1 (*) or to Ctrl2 (^#), or within a group (^†) between NitroSynapsin treatment vs. without (w/o) treatment (see Methods).