Fig. 5: Bulk RNAseq reveals differential and common gene expression signatures associated with AD in hippocampus and BLA of APP/Tau mice. | Molecular Psychiatry

Fig. 5: Bulk RNAseq reveals differential and common gene expression signatures associated with AD in hippocampus and BLA of APP/Tau mice.

From: Synaptic vulnerability to amyloid-β and tau pathologies differentially disrupts emotional and memory neural circuits

Fig. 5

A Number and Venn diagram illustrating the total, up and down differentially expressed transcripts in the hippocampus (HPC) and BLA of 9 month-old female APP/Tau mice (n = 9) compared to controls (WT, n = 11 for HPC; n = 10 for BLA). B Volcano plots showing the fold change of genes differentially expressed in the hippocampus and BLA of APP/Tau mice ( cut-off:   P value < 0.01, log2FC > 0.5). Some significant deregulated AD risk genes are indicated (see also Table 1). C Functional set enrichment of differentially expressed genes in APP/Tau hippocampus and BLA. The plots show the top significantly enriched pathways from the GWAS catalog (2019) (left) and GO Biological Process/Molecular Function (2021) (right) databases. Human orthologs were used in the analysis with GWAS catalog database. D Venn diagram illustrating the AD-associated genes identified previously by GWAS shared in the hippocampus of 6 and 9 month-old female APP/Tau and/or Tau mice. E Confocal microscope images (left) and quantification (right graphs) of percentage of GFAP (magenta; top images) and Iba1 (magenta; bottom images) areas, and Hoechst (blue) staining in CA1 hippocampus and BLA sections of female 9 month-old WT (n = 7), APP (n = 7), Tau (n = 4−5) and APP/Tau (n = 6−7) mice. Insets are magnified images of the indicated regions. Scale bars: 30 μm. Statistical analysis was determined by one-way ANOVA followed by Tukey’s post-hoc test. *P < 0.05, #P = 0.06 vs the indicated control group.

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