Fig. 2: Serotonin transporter inhibition enhances serotonin signals.
From: Coding principles and mechanisms of serotonergic transmission modes
A Simultaneous GRAB5HT and FSCV5HT recordings of evoked voltammetry and fluorescence ΔF/F0 responses before (green and red) and after (purple and pink) the bath application of 10 µm escitalopram (ECIT). B–C Peak amplitudes (Ctrl: 1.26 ± 0.19%; ECIT: 1.27 ± 0.08%, Z = 0.24, p = 0.85, n = 12 neurons from 6 animals) and decay time constants (Ctrl: 9.74 ± 1.25 s; ECIT: 23.84 ± 2.88 s, Z = 3.06, p < 0.001, n = 12 neurons from 6 animals) of GRAB5HT fluorescence ΔF/F0 responses before (green) and after (red) the bath application of ECIT. D–E Peak amplitudes (Ctrl: 25.67 ± 6.73 nM; ECIT: 38.22 ± 10.00 nM, Z = 2.66, p < 0.001, n = 9 neurons from 6 animals) and decay time constant (Ctrl: 3.09 ± 0.34 s; ECIT: 7.76 ± 1.14 s, Z = 2.66, p = 0.004, n = 9 neurons from 6 animals) of FSCV5HT voltammetric responses before (purple) and after (pink) the bath application of ECIT. Asterisks indicate p < 0.05 (Wilcoxon tests).
