Table 1 Sequences of primers used for qPCR.

From: CRISPRi-based screen of autism spectrum disorder risk genes in microglia uncovers roles of ADNP in microglia endocytosis and synaptic pruning

Primer description

Primer sequence (5′-3′)

GAPDH qPCR for

ATGCCTCCTGCACCACCAAC

GAPDH qPCR rev

GGGGCCATCCACAGTCTTCT

TREM2 qPCR for

CAGGGTATCAGCTCCAAACTC

TREM2 qPCR rev

CCAGAGCAGAACAAGGAGTC

ADNP qPCR for

GAGGATGTAGGACTGTGGGA

ADNP qPCR rev

TCTTCACTATGGACATTGCGG

TCF4 qPCR for

AACCATTACAGCACCTCTTCC

TCF4 qPCR rev

TGGAGAATAGATCGAAGCAAGTG

TCF7L2 qPCR for

AGAGGAAAAGGGACAAGCAG

TCF7L2 qPCR rev

GAAGTGAAAGGCAAGGATTTAGG

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