Fig. 6: Oxytocin and social rescue specifically affect emotion-related depressive-like phenotype.
From: Altered acetylcholine modulations and corticoaccumbal pathway in P11-linked social dysfunction
(A) Time in the light zone during the entire dark-light box test (0-6 min) for P11-WT or P11-KO following saline or oxytocin injection (0.5 mg/kg, i.p.). 2-way RM-ANOVA group effect F(1,24) = 65.02, P < 0.0001, drug F(1,24) = 0.056, P = 0.81, interaction F(1,24) = 1.574, P = 0.2217. (B) Time in the light zone during the entire dark-light box test with a WT-conspecific introduced in the light area (0-6 min) for P11-WT or P11-KO following saline or oxytocin injection. 2-way RM-ANOVA group effect F(1,24) = 43.56, P < 0.0001, drug F(1,24) = 8.034, P = 0.0092, interaction F(1,24) = 0.33, P = 0.5679. (C) Time sniffing the WT stimulus during the entire dark-light box test with a WT-conspecific introduced in the light area (0-6 min) for P11-WT or P11-KO following saline or oxytocin injection. 2-way RM-ANOVA group effect F(1,24) = 23.72, P < 0.0001, drug effect F(1,24) = 38.48, P < 0.0001, interaction F(1,24) = 4.960, P = 0.0356. (D-G) Time spent sniffing the stress (purple) or neutral (grey) stimulus in the emotional discrimination test for each segment of 2 min (0-2, 2-4, and 4-6 min) for P11-WT or P11-KO following oxytocin injection. Paired t-tests P11-WT-saline: 0-2 min P = 0.0004, 2-4 min P = 0.1156, 4-6 min P = 0.814; P11-WT-oxytocin: 0-2 min P = 1.10^-4, 2-4 min P = 0.1822, 4-6 min P = 0.0583; P11-KO-saline: 0-2 min P = 0.74, 2-4 min P = 0.30, 4-6 min P = 0.19, P11-KO-oxytocin: 0-2 min P = 0.0165, 2-4 min P = 0.17, 4-6 min P = 0.0026. (H) Discrimination index for EDT 2-way RM-ANOVA group effect F(1,24) = 28.71, P < 0.0001, drug F(1,24) = 3.647, P = 0.0677, interaction F(1,24) = 3.56, P = 0.0707. Unpaired t-test to 0.5 P11-WT saline P = 3.10^-8, P11-WT oxytocin P = 8.10^-6, P11-KO saline P = 0.463, P11-KO oxytocin P = 0.0036. (I) Schematic representation of the social rescue protocol between P11-WT (black) and P11-KO (red) female mice. (J) Time in the light zone during the entire dark-light box test (0-6 min) for P11-WT (black) and P11-KO (red) before and after social rescue. Unpaired t-tests Before P = 0.0001, After: P = 0.0502. (K) Time in the light zone during the entire dark-light box test with a WT-conspecific introduced in the light area (0-6 min) for P11-WT (black) and P11-KO (red) before and after social rescue. Unpaired t-tests Before P = 0.0072, After: P = 0.3388. (L) Time sniffing the WT stimulus during the entire dark-light box test with a WT-conspecific introduced in the light area (0-6 min) for P11-WT (black) and P11-KO (red) before and after social rescue. unpaired t-tests P11-WT versus P11-KO before: P = 0.029, after social mix: P = 0.98. (M, N) Time spent sniffing the stress (purple) or neutral (grey) stimulus in the emotional discrimination test for each segment of 2 min (0-2, 2-4, and 4-6 min) for P11-WT or P11-KO before (M) and after (N) social rescue. paired t-tests Before: P11-WT 0-2 min P = 4.10^-5, 2-4 min P = 0.0047, 4-6 min P = 0.38, 0-6 min P = 0.0002; P11-KO 0-2 min P = 0.76, 2-4 min P = 0.58, 4-6 min P = 0.86, 0-6 min P = 0.934, After: P11-WT: 0-2 min P = 0.0181, 2-4 min P = 0.3518, 4-6 min P = 0.3851, 0-6 min P = 0.0737; P11-KO: 0-2 min P = 0.0213, 2-4 min P = 0.1559, 4-6 min P = 0.083, 0-6 min P = 0.0603. Data are represented as mean ± SEM. Individual data are represented as dots when possible. Lines between histograms are used to represent time in a zone or interaction between conditions. *P < 0.05. $ P < 0.05 in comparison with 0.5 DI.
