Fig. 4: ELS increases the firing rates of a subpopulation of neurons in the BLAa in both male and female pre-juvenile mice and leads to a widespread increase in mean firing rates in BLAa of adolescent males. | Molecular Psychiatry

Fig. 4: ELS increases the firing rates of a subpopulation of neurons in the BLAa in both male and female pre-juvenile mice and leads to a widespread increase in mean firing rates in BLAa of adolescent males.

From: Early-life stress impairs development of functional interactions and neuronal activity within prefrontal-amygdala networks in vivo

Fig. 4: ELS increases the firing rates of a subpopulation of neurons in the BLAa in both male and female pre-juvenile mice and leads to a widespread increase in mean firing rates in BLAa of adolescent males.The alternative text for this image may have been generated using AI.

A (i) Distribution and median (IQR) of instant firing rates (Hz) of single units in the BLAa in control (grey, n = 41 units from N = 12 pups) and ELS (blue, n = 36 units from N = 12 pups) male pre-juvenile mice. (ii), Same as A (i) for Fano factor. B Logarithmic frequency distribution (bin width 0.3 s) of firing rates of single units in BLAa from both control (grey, n = 41 units, left) and ELS (blue, n = 36 units, right) pre-juvenile male mice. Note the bimodal distribution of firing rates in ELS mice, suggesting increased firing rates in a subpopulation of BLA neurons. C (i) Distribution and mean ± SD of the mean valley-to-peak time (ms) corresponding to the mAHP of the extracellular action potential (see diagram above) of single units in BLAa in control (grey, n = 41 units) and ELS (blue, n = 36 units) male pre-juvenile mice. Note the prominent decrease in ELS male mice. The diagram shows a representative action potential with key parameters highlighted in red. (ii) Same as C (i) for the full width at half maximum (FWHM, half-width (ms), see diagram in C (i)) of the extracellular action potential waveform. D (i) Distribution and median (IQR) of instant firing rates (Hz) of single units in the BLAa in control (grey, n = 34 units from N = 9 pups) and ELS (magenta, n = 24 units from N = 10 pups) female pre-juvenile mice. (ii), Same as C (i) for Fano factor. E Logarithmic frequency distribution (bin width 0.3 s) of firing rates of single units in BLAa from both control (grey, n = 34 units, left) and ELS (magenta, n = 24 units, right) pre-juvenile female mice. Note the bimodal distribution of firing rates in ELS mice, suggesting increased firing rates in a subpopulation of BLA neurons. F (i) Distribution and mean ± SD of the mean valley-to-peak time (ms) of single units in BLAa in control (grey, n = 36 units) and ELS (magenta, n = 24 units) female pre-juvenile mice. (ii) Same as F (i) for the full width at half maximum (ms) of the extracellular action potential waveform. ****p < 0.0001, Mann-Whitney test. G (i) Distribution and median (IQR) of instant firing rates (Hz) of single units in the BLAa in control (grey, n = 47 units from N = 10 pups) and ELS (blue, n = 77 units from N = 11 pups) male adolescent mice. Mann-Whitney test, ***p = 0.0004. (ii), Same as G (i) for Fano factor. Mann-Whitney test, **p = 0.0026. H Logarithmic frequency distribution (bin width 0.3 s) of firing rates of single units in BLAa from both control (grey, n = 47 units, left) and ELS (blue, n = 77 units, right) adolescent male mice. I (i) Distribution and median (IQR) of the mean valley-to-peak time (ms) corresponding to the mAHP of the extracellular action potential of single units in BLAa in control (grey, n = 47 units) and ELS (blue, n = 77 units) male adolescent mice. Unpaired t-test, **p = 0.0041. (ii) Distribution and mean ± SD for the full width at half maximum (FWHM, half-width (ms), see diagram in C (i)) of the extracellular action potential waveform. J (i) Distribution and median (IQR) of instant firing rates (Hz) of single units in the BLAa in control (grey, n = 109 units from N = 14 pups) and ELS (magenta, n = 29 units from N = 8 pups) female adolescent mice. (ii), Same as J (i) for Fano factor. Mann-Whitney test, ****p < 0.0001. K Logarithmic frequency distribution (bin width 0.3 s) of firing rates of single units in BLAa from both control (grey, n = 109 units, left) and ELS (magenta, n = 29 units, right) adolescent female mice. L (i) Distribution and median (IQR) of the mean valley-to-peak time (ms) of single units in BLAa in control (grey, n = 109 units) and ELS (magenta, n = 29 units) female adolescent mice. Mann-Whitney test, *p = 0.0225 (ii) Distribution and mean ± SD of the full width at half maximum (ms) of the extracellular action potential waveform. Unpaired t test, ***p = 0.0006.

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