Fig. 1: Chemogenetic activation of distinct thalamic nuclei within three different thalamocortical circuits impairs sleep. | Molecular Psychiatry

Fig. 1: Chemogenetic activation of distinct thalamic nuclei within three different thalamocortical circuits impairs sleep.

From: Hyperactivation of distinct thalamic nuclei differentially impairs sleep physiology in rats

Fig. 1: Chemogenetic activation of distinct thalamic nuclei within three different thalamocortical circuits impairs sleep.The alternative text for this image may have been generated using AI.

A Schematic of the experimental design and dosing scheme for the chemogenetic manipulation. B Example of the EEG signal in the frontal and parietal cortex and the accelerometer vector in the different physiologic states (NREM; REM). (C) hM3Dq expression in CamKII cells on the MDT and the respective quantification of the area of the virus expression. Time spent in each physiological state of MDT-hM3Dq, (D) wake, (E) NREM light sleep, (F) NREM deep sleep and (G) REM. H Number of wake/NREM transitions of MDT-hM3Dq rats. I–N plots for the VPT-hM3Dq animals. O–T Plots for the VMT-hM3Dq animals. U–Y Plots for the no virus controls animals. Vehicle (blue), DCZ 0.1 mg/kg (red); N = 12 MDT-hM3Dq rats; N = 9 VPT-hM3Dq rats; N = 10 VMT-hM3Dq rats; N = 12 no virus controls rats. Statistically significant clusters (p < 0.05) are indicated as bars on top based on a paired cluster-based permutation test. The x-axis labels correspond to the end time of each one-hour bin (e.g. 12:00 AM time bin (second time bin) refers to data collected between 11:00 AM and 12:00 AM); only a fraction of time bins are labeled to enhance readability.

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