Fig. 2: GluN1-Ab induces intrinsic hypercoupling of CA1 PNs.

A Schematic representation of the STTC and PCC quantifications of coupling between CaT-trains of putative neuron (PN) pairs. B Distribution of STTC indices, considering all PN pairs (tiling window of 0.2 sec). For boxplots, only pairs with positive (pos.) coupling were considered. C Increased coupling of PN pairs with significantly high STTC (see Supplementary Fig. 3A,B) under GluN1-Ab. Same format as in (B). D Example STTC matrices (re-ordered) of all (top row) and significantly (bottom row) coupled PN pairs from two individual FOVs. E Relationship between STTC and Euclidean somatic distance of PNs. Left, schematic of inter-PN distance used for quantification of the relationships shown in the right panels. For example, for 50–100 μm, for each PN, its average coupling with neurons locating within this range are computed. Radius: ca. 50 to 350 μm. (F, G) Increased neuronal coupling under GluN1-Ab at various timescales. The measures were quantified at multiple tiling windows of STTC (F) or standard deviation of Gaussian kernel used for smoothing CaT-trains in PCC (G). Curves represent mean ± SEM across mice. Boxplots show median and interquartile range; dots represent individual mice. Sample sizes: (B–C, E–G) n = 9 mice per group (total cells: 4279 Ctrl-Ab, 3200 GluN1-Ab). Statistical comparisons: Mann-Whitney U tests (B, C) or permutation tests (E–G); see Supplementary Table 1.