Fig. 4: GluN1-Ab leads to higher transmission fidelity and restructures CA1 spatiotemporal activity patterns around aberrant ensembles.
A Schematic representation of inter-neuronal transmission metrics for a single potential transmitter (PNT) to a potential receiver (PNR) based on the CaT-onset trains of putative neurons (PNs). Reliability: fraction of successfully transmitted CaTs; Latency: average time delay of this transmission; Efficacy: average number of CaTs transmitted to PNR, upon PNT activation. Bias denotes the randomization-based value of each metric. For each PNT, its value for a given metric is determined as the mean or median over its outgoing connections (to PNRs), i.e. over its corresponding row in the depicted transmission matrix. B Higher reliability and reduced latency of individual PNs’ transmission to others. Per metric, PNTs with significant level (e.g. of reliability), as compared to randomized data, were considered (sig. PNs). Transmission window was set to 0.6 s. C Transmission metrics of sig. PNs at different plausible timescales. D Schematic representation of similarity quantification between a pair of SE patterns using the matching index (MI). E, F Excessive similarity between SE spatial-pattern pairs with significantly high MIs, compared to similarity between spatially randomized SE patterns. E Distribution of significant MIs. Same format as in Fig. 1E. F Top, boxplots of summary statistics of significant MIs. Bottom, significant MIs as a function of minimum fraction of active PNs in each SE pattern. Quartiles of the SE-detection thresholds of all mice: Q1 ≈ 3.9, Q2 ≈ 4.3, Q3 ≈ 4.9%. G Excessive similarity between the spatial patterns, obtained by a non-overlap binning of entire recording time using a relatively short, fixed bin-size (∼1 s). The binned pattern pairs with significantly high MIs were shown, similarly to (E). Inset: boxplot of corresponding median values. H Similarly to inset in (G), but as a function of bin-size. (I–K) Functional and transmission metrics of PNs belonging to the neuronal ensembles in Fig. 3G–I, analyzed at various timescales. Same format as in Fig. 2F. Transmission window was set to 1.2 s in (K). Curves represent mean ± SEM across mice. Boxplots show median and interquartile range; dots represent individual mice. Sample sizes: (B, C, E–K) n = 9 mice/group (total cells: 4279 Ctrl-Ab, 3200 GluN1-Ab). Statistical comparisons: two-sample t-tests (asterisks in I–K), Mann-Whitney U tests (B, F[top], G, asterisk in H), or permutation tests (C, F [bottom], H-K); see Supplementary Table 1.
