Fig. 6
Over-expression of Notch signaling leads to increased IL-4 production by Tfh cells but not lung-resident CD4+ T cells. a Diagram of experimental set-up: IL4KN2ERcreRosa26Stop-flox-NICD-GFP (n = 4) mice were injected with tamoxifen once every 24 h for 3 days starting on day 3. On day 0 mice were infected with N. brasiliensis and mediastinal lymph nodes were harvested on day 9. b Representative flow plots of CD4+ T cells isolated from the lung. Gates shown represent cells with endogenous Notch signaling (CD4+ GFP-) and cells over-expressing NICD (CD4+ GFP+). IL-4 protein (huCD2) expression was analyzed and quantified as a percent of the gated populations. c Contour plots of Tfh (PD1+ CXCR5+) cells isolated from mediastinal lymph nodes. IL-4 protein (huCD2) expression was assessed and quantified in Tfh cells with endogenous levels of Notch signaling (CD4+ , PD1+ , CXCR5+ , GFP-) and Tfh cells over-expressing NICD (CD4+ , PD1+ , CXCR5+ , GFP+). Error bars represent +/- SEM. Data is representative of four independent experiments with n = 3–5 mice per group. ***P < 0.001 (paired two-tailed t-test)
