Fig. 3
From: Modulation of bacterial metabolism by the microenvironment controls MAIT cell stimulation
Bacterial riboflavin-related synthetic antigens activate MAIT cells in a dose-dependent manner. a, b Dose-dependent IFNγ response of the MAIT cell clone (a) SMC3 and (b) MRC25 after stimulation with A375.MR1 cells and RL-6,7-diMe (diamonds), 5-OP-RU (circles), 5-OE-RU (squares), or in the absence of antigen (ns, not stimulated). c Log10EC50 values of RL-6,7-diMe (diamonds), 5-OE-RU (squares), and 5-OP-RU (circles) inducing half maximum IFNγ release by MAIT cell clones as in a, b. Data show mean ± sd of duplicates and are representative of two independent experiments. d Flow cytometry assessing TCR downregulation on MAIT cells (CD3+Vα7.2+CD161hi) present in PBMCs of a representative donor after co-culture with A375.MR1 and RL-6,7-diMe (50 µM), 5-OE-RU (0.5 µM), 5-OP-RU (5 nM) or E. coli extract (5 × 107 CFU/mL). e, f Median fluorescence intensity (MFI) of the TCR on MAIT cells (Vα7.2+CD161hi as in d of another donor in response to varying doses of e 5-OP-RU (circles), 5-OE-RU (squares), RL-6,7-diMe (diamonds) or f E. coli extract. One representative out of five experiments is shown
