Fig. 4
From: Modulation of bacterial metabolism by the microenvironment controls MAIT cell stimulation
Multiple populations of ex vivo MAIT cells respond to riboflavin-related antigens. MAIT cell response of one healthy donor after PBMC stimulation for 24 h with A375.MR1 cells and a–c RL-6,7-diMe, d–f 5-OE-RU, g–i 5-OP-RU, j, k E. coli extract or l medium (unstimulated). The response to EC50 and ED50 doses of each antigen are presented in the middle panels (b, e, h, k). t-SNE analysis of CD3+Vα7.2+CD161hi MAIT cells after down-sampling and concatenation of all conditions. Warm color regions of the t-SNE map depict regions of higher cell density. Experiments were performed twice with comparable results in two different donors
