Fig. 5
From: Modulation of bacterial metabolism by the microenvironment controls MAIT cell stimulation
Clusters of ex vivo-stimulated MAIT cells according to their phenotypic profile. FlowSOM analysis of MAIT cells from one healthy donor after PBMC stimulation with A375.MR1 cells and RL-6,7-diMe, 5-OE-RU, 5-OP-RU, (EC50 concentrations), and E. coli extract (107 CFU/mL, ED50), or medium (unstimulated) for 24 h and 72 h. a Minimal spanning tree illustrating 25 nodes containing MAIT cells stimulated with various doses of antigens and E. coli extract. Radar charts show expression of markers defining each node. b, c Expression of indicated surface markers on unstimulated and antigen stimulated MAIT cells after b 24 and c 72 h in the main node is shown. Minor nodes are shown in Supplementary Fig. 6. Numbers indicate main nodes defined by the semi-supervised FlowSOM algorithm in a. Pie charts indicate the median fluorescence intensity of markers and node size corresponds to the number of MAIT cells in each population. Experiments were performed twice with comparable results in three healthy donors
