Fig. 1

T_H17-associated ileal hyperplasia without neutrophil increase in juvenile mice. a Bigenic mouse breeding schematic with ages and age-ranges (“Weanling age” 23 ± 2 days; “Juvenile age” 50 ± 7 days; “Early Adult age” 100 ± 7 days; “Late Adult age” 150 ± 7 days; “Juvenile age-range” between weanling and juvenile ages) of mice analyzed in our study. b Mouse weights for WT, 3A9 and Bigenic mice normalized to age and sex (Weight Z-score). Weight at weaning (WT n = 491, 3A9 n = 155, Bigenic n = 338) and at juvenile (WT n = 316, 3A9 n = 131, Bigenic n = 147) ages. c Percent of live circulating cells that are TCRβ⁺CD4⁺CD8^–Foxp3^–CD44⁺CD62L^– (CD4⁺T_EM) for WT, 3A9 and Bigenic mice. Shown at weanling (WT n = 271, 3A9 n = 36, Bigenic n = 118) and juvenile (WT n = 28, 3A9 n = 18, Bigenic n = 37) ages. Correlation to weight Z-score at time of observation for Juvenile Bigenic cohort shown. FACS plot representative of CD4⁺T_EM as a percent of TCRβ⁺CD4⁺CD8^–Foxp3^– cells. d Average Ileal crypt depths for juvenile WT (n = 21), 3A9 (n = 12) and Bigenic (n = 19) mice. Representative crypts lengths shown for WT (104, 98 μm), 3A9 (107, 122 μm) and Bigenic (146, 162 μm) ileal tissue. e Numbers of TCRβ⁺CD4⁺CD8^–Foxp3^–IFNγ⁺IL-17A^– (T_H1) and TCRβ⁺CD4⁺CD8^–Foxp3^–IFNγ^–IL-17A⁺ (T_H17) cells accumulating in juvenile WT, 3A9 and Bigenic mice. Numbers from mLN (WT n = 24, 3A9 n = 19, Bigenic n = 21) and ileum (WT n = 7, 3A9 n = 12, Bigenic n = 14) and representative FACS plot shown from Bigenic ileum. f Serum concentrations of T_H17-associated cytokines from juvenile WT (n = 6), 3A9 (n = 6) and Bigenic (n = 13) mice. Cytokines IL-6, CCL20, IL-22, IL-17A, IL-17F, and TNFα shown. g Percent of mucosal surface with neutrophil immunohistochemical stain from WT (n = 21), 3A9 (n = 10) and Bigenic (n = 18) ileal tissue sections. Representative neutrophil IHC section from Bigenic ileum with neutrophil (green) and counter stain (orange) detection indicated. Scale bar reflects 100 μm