Fig. 2 | Mucosal Immunology

Fig. 2

From: Human intestinal pro-inflammatory CD11chighCCR2+CX3CR1+ macrophages, but not their tolerogenic CD11c−CCR2−CX3CR1− counterparts, are expanded in inflammatory bowel disease

Fig. 2Fig. 2

Characterization of human intestinal macrophage subsets. a Human colonic macrophage (Mϕ) subsets were identified as in Fig. 1d and characterized for the expression of CD14, CD64, HLA-DR and SIRPα. Shaded histograms denote the expression for each marker on the CD14−CD64− fraction. b Mϕ subsets were further characterized for the expression of Mϕ-associated markers including CCR2, CD40 and CX3CR1; c as well as CD86, CD206 and CD163. Given the differences in the autofluorescence displayed by the different Mϕ subsets (Fig. 1e), the percentage of positive cells for each marker on each given Mϕ subset was determined based on their specific Fluorescence Minus One (FMO). One-way ANOVA repeated measures with Tukey correction was applied in all cases. p-Values < 0.05 were considered significant (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)

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