Fig. 3 | Mucosal Immunology

Fig. 3

From: Human intestinal pro-inflammatory CD11chighCCR2+CX3CR1+ macrophages, but not their tolerogenic CD11c−CCR2−CX3CR1− counterparts, are expanded in inflammatory bowel disease

Fig. 3

Human circulating monocyte subsets. a Total circulating monocytes were identified within peripheral blood mononuclear cells from healthy controls and divided into classical (CD14+CD16−), intermediate (CD14+CD16+) and non-classical (CD14−CD16+). b Monocyte subsets were further characterized for the expression of HLA-DR, CX3CR1, CD40 and CCR2. Shaded histograms denote the expression for each marker on the HLA-DR− fraction. Pooled data from several independent experiments are shown in c. d Human colonic macrophages (Mϕ) were divided into subsets based on the expression of CD11c (CD11chigh, CD11cdim, CD11clow), CCR2 (CCR2+, CCR2−) and CX3CR1 (CX3CR1+, CX3CR1−). Further analysis of the expression for these markers was determined within each specific subset. Results are representative from five independent experiments performed with similar results. One-way ANOVA repeated measures with Tukey correction was applied in c. p-Values <0.05 were considered significant (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)

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