Fig. 4 | Mucosal Immunology

Fig. 4

From: Human intestinal pro-inflammatory CD11chighCCR2+CX3CR1+ macrophages, but not their tolerogenic CD11c−CCR2−CX3CR1− counterparts, are expanded in inflammatory bowel disease

Fig. 4Fig. 4

CD11chigh macrophages produce larger amounts of IL-1β while CD11c− macrophages secrete IL-10, both in resting conditions and after LPS stimulation. a Human colonic macrophage (Mϕ) subsets were identified as in Fig. 1d and their spontaneous production of intracellular IL-1β, TNFα and IL-10 determined. Positive and negative gatings for each cytokine on each subset were determined by comparison with their respective Fluorescence Minus One (FMO). b Intracellular production of IL-1β and c IL-10 was further determined for each Mϕ subset following 18 h culture of LPMC in the presence/absence of lipopolysaccharide (LPS). One-way ANOVA repeated measures with Tukey correction was applied in a, while two-way ANOVA repeated measures with Sidak correction was applied in b, c. p-Values <0.05 were considered significant (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)

Back to article page