Fig. 6

CD11c⁻ macrophages are induced by the colonic mucosa. a Macrophage (Mϕ) colonic subset composition was determined as in Fig. 1d within fresh lamina propria mononuclear cells (LPMC), as well as after 18-h culture in complete medium (Basal). b Mϕ subset composition was also determined following 18-h culture of LPMC in the presence/absence of LPS. c GM-CSF-derived Mϕ were generated, following 7 days differentiation of CD14⁺ monocytes, in the presence/absence of cell-free colonic biopsy culture supernatant (Bx-SN). The proportion of CD11c⁻ macrophages was determined at the end of the culture, as well as d the intracellular cytokine profile (IL-1β and IL-10) of both the CD11c⁺ and the CD11c⁻ fractions. Two-way ANOVA repeated measures with Sidak correction was applied in a, b and d, while paired t-test was applied in c. p-Values <0.05 were considered significant (*p < 0.05; **p < 0.01; ***p < 0.001). Note that the 18-h plot in a and the 18-h basal culture plot in b are the same, as in a the plot is compared with their paired non-cultured cells, while in b the plot is compared with the paired 18-h culture in the presence of LPS