Fig. 7 | Mucosal Immunology

Fig. 7

From: Human intestinal pro-inflammatory CD11chighCCR2+CX3CR1+ macrophages, but not their tolerogenic CD11c−CCR2−CX3CR1− counterparts, are expanded in inflammatory bowel disease

Fig. 7

The inflamed colon from IBD patients displays an increased capacity to recruit circulating monocytes in a CCR2-dependent manner. a Colonic biopsy culture supernatants from healthy controls (HC), patients with active ulcerative colitis (aUC), quiescent ulcerative colitis (qUC), active Crohn's disease (aCD) or quiescent Crohn's disease (qCD) were further evaluated on transwell inserts for their capacity to recruit circulating CD14+ classical monocytes from healthy controls. b Prior to performing the migration, monocytes were pre-incubated with anti-CCR2 (or its respective isotype) to evaluate whether monocyte migration towards the colonic culture supernatants was CCR2 dependent. Dotted lines denote basal, or spontaneous migration, of monocytes towards the lower chamber with unconditioned medium. One-way ANOVA with Tukey correction was applied in a, while two-way ANOVA repeated measures with Sidak correction was applied in b. p-Values < 0.05 were considered significant (*p < 0.05; ***p < 0.001; ****p < 0.0001)

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