Fig. 1
DLL4-enriched permissive histone mark H3K4me3 around Foxp3 promoter and consensus non-coding sequences during iTreg differentiation. a 2 × 106 of naive CD4 T cells were skewed toward Th0 or iTreg differentiation with or without DLL4 stimulation in vitro. Chromatin immunoprecipitation were performed to detect H3K4me3 around Foxp3 promoter, consensus non-coding sequence (CNS)1, CNS2, CNS3 after 72 h. b Changes of H3K4me and H3K4me3 by DLL4 stimulation during iTreg differentiation was detected at 48 h of skewing. c H3K4me3 kinetics at Foxp3 CNS1 after 6 h, 24 h, 48 h and 72 h post iTreg differentiation were measured with or without DLL4 stimulation in vitro. Data represent mean ± SEM. Data were from one experiment representative of two to three experiments. * P < 0.05; ** P < 0.005; *** P < 0.0005; NS, no significance (unpaired two-tailed t-test)
