Fig. 5
Smyd3 deletion in T cells exacerbated RSV immunopathology and CD4 T cells cytokine production in vivo during RSV infection. a Smyd3 expression in primary lymphoid organ: thymus, secondary lymphoid organ: spleen, and non-lymphoid tissue: lung from uninfected wild-type B6 mice were measured. N = 4. b Smyd3 expression in uninfected naive mice and i.t. RSV infected mice in lung at 4 dpi and 6 dpi were measured. N = 4. c CD4-specific Smyd3 knockout (Cre + Smyd3 cKO) and Cre− littermate control were intratracheally infected with RSV. The residual quantity of RSV surface glycoprotein (RSV-G) expression in lung were measured at 6 dpi and 8 dpi. N = 5~6. d Periodic acid-Schiff (PAS) staining of formalin-fixed lung section from 8 dpi. Bar, 100 μm. ↑ indicates the detection of mucin. N = 5~6. e Percent of viable CD25 + Foxp3 + Treg in lung were gated and quantified at 8 dpi. f mLN cells were harvest at 8 dpi and re-stimulated with RSV for 48 h. IL-17A in the supernatant were measured. g mLN cells were collected at 8 dpi and re-stimulated with RSV for 48 h. IFN-γ in the supernatant were measured. h mLN cells were harvest at 8 dpi and re-stimulated with RSV for 48 h. IL-5 in the supernatant were measured. i mLN cells were harvest at 8 dpi and re-stimulated with RSV for 48 h. IL-10 in the supernatant were measured. Data represent mean ± SEM. Data were from one experiment representative of two experiments with four to six mice per group, with samples from each mouse processed and analyzed separately. *P < 0.05; **P < 0.005; ***P < 0.0005; ND, not-detectable; NS, no significance (unpaired two-tailed t-test)
