Fig. 3

Replacement kinetics, calprotectin expression and proliferation of DC subsets in vivo. a Flow cytometry plots depicting donor-derived vs. recipient-derived cells among the main hSI DC subsets, CD14⁺CD11c⁺ Mf and stromal cells in the graft tissue at 6 weeks post-transplantation, representative of five subjects. b Distribution of recipient-derived cells among cell populations in the graft at 3 (N = 7) and 6 weeks (N = 5) post-transplantation. * P < 0.05 ** P < 0.01 (RM-ANOVA). c Expression of Ki67 within the main DC subsets (N = 6) in steady-state hSI (ANOVA). d Calprotectin expression in donor-derived vs. recipient-derived cells in the graft 3 weeks after transplantation (N = 3). ** P < 0.01 (RM-two-way-ANOVA). e Co-expression of calprotectin and Ki67 (red) within SIRPα⁺ DCs in steady-state hSI (N = 5). Gates were set based on isotype-matched control antibodies (dark-grey contour represents isotype control for Ki67). * P < 0.05 (t-test)