Fig. 5

Flt3^- APCs are enriched for monocyte gene signatures according to RNA-seq data. a Principal component analysis on RNA-seq data generated from six subsets of hSI cells of the classical DC phenotpye and CD14⁺CD11c⁺ Mf, sorted as in Fig. 4c (N = 2). b K-means (k = 4) clustering of RNA-seq data for the cell subsets. c Overlap of clusters 1 and 2/3 from k-means clustering of RNA-seq data with core gene signatures generated from published data for human blood monocyte and cDC2 populations, respectively. d Gene expression values of calprotectin subunits S100A8, S100A9, and CD5 plotted vs. gene expression values of FLT3 generated from RNA-seq. # indicates two visually overlapping data points