Fig. 7 | Mucosal Immunology

Fig. 7

From: Transcriptional profiling reveals monocyte-related macrophages phenotypically resembling DC in human intestine

Fig. 7

Only Flt3+ DCs efficiently prime naïve T cells. a Flow cytometric quantification of proliferation (CFSE dilution) and CD25 expression in allogeneic CD4+ T cells after coculture with the different subsets of tissue DCs and CD14+CD11c+ Mf (N = 4). Tissue cells were sorted as described in Fig. 4c. IL-2 concentration in coculture supernatants from day 6 was analyzed by Bio-Plex Assay. (b) Abundance of T-bet+ and (e) IL17A+ CD4+ T cells after PMA/ionomycin stimulation. (c) IFNγ and (d) TNF concentrations in coculture supernatants were analyzed by Bio-Plex Assay (ANOVA)

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