Fig. 1
From: Chronic intestinal inflammation in mice expressing viral Flip in epithelial cells
vFlipIEC-tg mice spontaneously develop severe intestinal inflammation. a Strategy for generating vFlipIEC-tg mice. Rosa26.vFLIP knockin mice were crossed to VillinCre mice to generate mice constitutively expressing vFlip in IECs. b Western Blot analysis of GFP using small intestinal IEC lysates from indicated mice. Actin was used as a loading control. c Representative pictures of GFP fluorescence staining of small intestinal cross sections from indicated mice. Scale bar 75 µm. d Representative endoscopic pictures from the small intestine of indicated mice. e Transcriptional analysis of Tnfa (n ≥ 10) and S100a9 (n ≥ 8) in the small intestine of control and vFlipIEC-tg mice. Values are shown + SD and were calculated relative to control mice. Hprt was used as internal standard. Data were pooled from 4 individual experiments, **p < 0.01. f Representative pictures of H&E stained small intestinal cross sections from indicated mice. Scale bar upper panel 500 µm, lower panel 100 µm. g Representative pictures of immunohistochemical stainings of small intestinal cross sections from indicated mice using antibodies against F4/80, CD11c and CD4. Scale bar 75 µm
