Fig. 2
From: Chronic intestinal inflammation in mice expressing viral Flip in epithelial cells
vFlipIEC-tg mice are characterized by reduced Paneth cell numbers and increased cell death. a Representative pictures of H&E and immunohistochemical Lysozyme staining of small intestinal cross sections of indicated mice. Scale bar 50 µm. b Transcriptional analysis of Ang4 (n = 11) and Lyz (n = 11) in the small intestine of indicated mice. Values are shown + SD and were calculated relative to control mice. Hprt was used as internal standard. Data were pooled from 4 individual experiments, **p < 0.01, ***p < 0.001. c Representative pictures of TUNEL/Cleaved Caspase-3 doublestaining of small intestinal cross sections from indicated mice. Scale bar upper panel: 75 µm, lower panel: 50 µm. d Quantification of cell death of IECs located at the villus or the crypt area. Villi: n(control) = 5, n(vFlipIEC-tg) = 10, crypts: n(control) = 8, n(vFlipIEC-tg) = 13, ***p < 0.001. e Transcriptional analysis of Rip3 (n ≥ 9) and Mlkl (n ≥ 9) in the small intestine of indicated mice. Values are shown + SD and were calculated relative to control mice. Hprt was used as internal standard. Data were pooled from 3 individual experiments, ***p < 0,001. f Western Blot analyses of small intestinal IEC lysates from indicated mice using antibodies against RIP3, Caspase-3, its cleaved forms and MLKL. Actin was used as a loading control. g Quantitative proteome analysis of known and putative STAT1 target genes in small intestinal epithelial lysates of control and vFlipIEC-tg mice (n = 3). Values are shown + SD and were calculated relative to control mice, p < 0.05 for all target genes between both groups
