Fig. 2 | Mucosal Immunology

Fig. 2

From: Sustained protective immunity against Bordetella pertussis nasal colonization by intranasal immunization with a vaccine-adjuvant combination that induces IL-17-secreting TRM cells

Fig. 2

A TLR2-STING agonist combination is an effective adjuvant and promotes antigen-specific Th1 and Th17 responses to a model antigen in vivo. Mice were injected in the footpad with PBS, KLH (1 μg/mouse), KLH + c-di-GMP (KLH + GMP; 10 μg/mouse), KLH + LP1569 (KLH + LP; 50 μg/mouse) or KLH + LP-GMP. Seven days later, the popliteal lymph nodes were isolated and cells were restimulated with increasing concentrations of KLH (2, 50, 100 μg/ml). After 72 h, KLH-specific cytokine production in the supernatants was assessed by ELISA. Results are representative of two experiments and mean ± SEM values for 3 mice per group. **P < 0.01, ***P < 0.001 vs. PBS; ++P < 0.01, +++P < 0.001 vs. KLH; ◆◆P < 0.01, ◆◆◆P < 0.001 vs. KLH + GMP; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. KLH + LP by two-way ANOVA with the Bonferroni post-test

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